Interstrand-cross-link repair
WebERCC1 (Excision Repair Cross-Complementing Group 1) is a mammalian endonuclease that incises the damaged strand of DNA during nucleotide excision repair and interstrand crosslink repair. Ercc1-/Δ mice, carrying one null and one hypomorphic Ercc1 allele, have been widely used to study aging due to accelerated aging phenotypes in numerous … WebRemoval of a DNA interstrand crosslink (a covalent attachment of DNA bases on opposite strands of the DNA) and restoration of the DNA. DNA interstrand crosslinks occur when …
Interstrand-cross-link repair
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WebJul 1, 2011 · DNA interstrand cross-links (ICLs) are toxic DNA lesions whose repair in S phase of eukaryotic cells is incompletely understood. In Xenopus egg extracts, ICL … WebJul 4, 1995 · The combination of cis-diamminedichloroplatinum(II) (CDDP) and 7-ethyl-10-[4-(I -piperidino)-I -piperidino]carbonyloxycamptothecin (CPT-I I), a topoisomerase-l inhibitor, has been shown to be synergistic in vitro and clinically active against several human cancers refractory to chemotherapy. To elucidate the mechanism of the synergistic cytotoxicity of …
WebApr 11, 2024 · The compound generates a specific form of DNA damage, known as an “interstrand cross-link,” which is highly toxic to cells. Dr. Herzon said their new findings bolster their mechanistic model and suggests KL-50 may be extensible to other tumor types harboring specific DNA repair defects.
WebJul 29, 2024 · The HMCES protein forms a covalent DNA-protein cross-link (DPC) with abasic (AP) sites in ssDNA, and the resulting HMCES-DPC is thought to suppress double-strand break formation in S phase. However, the dynamics of HMCES cross-linking and whether any DNA repair pathways normally include an HMCES-DPC intermediate … WebIn contrast, the percentage repair (unhooking) of DNA interstrand crosslinks was much greater in the group of treated patients. At 24 h in the 36 newly diagnosed patient tumour …
In genetics, crosslinking of DNA occurs when various exogenous or endogenous agents react with two nucleotides of DNA, forming a covalent linkage between them. This crosslink can occur within the same strand (intrastrand) or between opposite strands of double-stranded DNA (interstrand). These adducts interfere with cellular metabolism, such as DNA replication and transcription, triggering cell death. These crosslinks can, however, be repaired through excision or recombinati…
WebDNA is subject to a wide variety of endogenous and environmentally induced modifications, including base oxidation, deamination, depurination and formation of interstrand cross-links (1). The ... 2k14名单补丁怎么用WebJun 24, 2011 · DNA interstrand crosslink repair and cancer Nat Rev Cancer. 2011 Jun 24;11(7):467-80. doi: 10.1038/nrc3088. Authors Andrew J Deans 1 , Stephen C West. … 2k14 姚明动作WebJan 1, 2024 · Facile preparation of model DNA interstrand cross-link repair intermediates using ribonucleotide-containing DNA DNA Repair (Amst) , 111 ( 2024 ) , Article 103286 , … 2k14怎么下载WebIntrauterine growth restriction (IUGR) and preeclampsia (PE) are placental pathologies known to complicate pregnancy and cause neonatal disorders. To date, there is a limited number of studies on the genetic similarity of these conditions. DNA methylation is a heritable epigenetic process that can regulate placental development. Our objective was … 2k14名单放哪里WebAug 16, 2012 · DNA is constantly challenged by agents that can inflict structural damage either on one or both strands of the DNA duplex. Lesions affecting one strand present a limited challenge, as the complementary undamaged DNA strand provides the repair machinery with a template to accurately restore the original sequence in the damaged … 2k14曾凡博面补Preparations of the following plasmids containing site-specific crosslinks were performed as previously described: pICLPt; (ref. 2), pICL-lacOPt; (ref. 7), and pICLAP and pICL-lacOAP; (ref. 1). In brief, purified cisplatin- or AP-crosslinked oligonucleotide duplexes comprising Pt_Top and Pt_Bottom or … See more Preparation of pDPC2×lead was performed as previously described3,28. In brief, oligonucleotide DPC_Fluoro_dC was ligated into a parental plasmid nicked with Nt.BbvCI and then … See more Replication reactions were stopped at the indicated time points with 10 volumes of Stop Solution A (5% SDS, 80 mM Tris-HCl (pH 8.0), 0.13% phosphoric acid, 10% Ficoll, and 0.5% … See more All animal work was approved by the Harvard Medical Area Standing Committee on Animals (HMA IACUC Study ID … See more Rabbit polyclonal antibodies raised against the following X. laevis proteins have been previously described: BRCA131; CDC45 and MCM732; CLASPIN33; CUL2, LRR1 and TRAIP23; FANCM28; MCM6 and NEIL31; MCM1034; … See more 2k14怎么全屏WebInterstrand crosslink (ICL) recognition by FANCM and associated proteins leads to the recruitment of the Fanconi anaemia (FA) core complex and monoubiquitylation (Ub) of … 2k14有多大